ChromasPro Technelysium Pty Ltd
winget install --id=Technelysium.Chromas.Pro -e ChromasPro is suitable for DNA sequence assembly projects up to a few megabases, and basic sequence editing and analysis. It is able to assemble data from Sanger sequencers such as ABI, and 454 and Illumina next-generation sequencers, with up to 1,000,000 sequences if 8 Gb RAM is available. ChromasPro has the following features: - Open sequences in Applied Biosystems .ab1, Staden Chromatogram (SCF and ZTR), 454 SFF, FASTA, FASTQ, EMBL, GenBank, SwissProt, GenPept, GCG RSF and plain text formats. - Save sequences in .ab1, .scf, .fasta and .fastq formats. - Assemble overlapping sequences using a graphical interface, generate a consensus and highlight ambiguities for editing. - Use quality data to automatically remove low quality sequence for improved sequence assembly. - Use a reference sequence as a scaffold for assembly. - Generate restriction site and fragment maps, and list cutters, non-cutters and fragments. - Map open reading frames, aided by a G+C frame plot, and translate ORFs with one click. - Print chromatograms, restriction site and fragment maps, and open reading frame maps. - Perform nucleotide and protein BLAST searches through the NCBI web site. - Perform multiple alignments by interfacing with ClustalW. - Reverse & complement sequences and chromatograms. - Search for sequences by exact matching or optimal alignment. - Display translations when editing nucleotide sequences. - Perform reverse translations and plot nucleotide degeneracy. - Plot hydrophilicity and antigenicity of proteins. - Copy an image of a chromatogram section for pasting into documents or presentations.
ChromasPro is a DNA sequence assembly and analysis tool designed to facilitate projects ranging from small-scale sequencing efforts to large assemblies of up to a few megabases in size. It supports the assembly of data generated by Sanger sequencers (such as ABI), 454, and Illumina next-generation sequencers, capable of handling up to 1,000,000 sequences with sufficient RAM (8 Gb or more).
Key features include:
- Support for importing a wide range of file formats, including .ab1, SCF, ZTR, SFF, FASTA, FASTQ, EMBL, GenBank, SwissProt, and plain text.
- Export capabilities in .ab1, .scf, .fasta, and .fastq formats.
- A graphical interface for assembling overlapping sequences, generating consensus sequences, and highlighting ambiguities for manual editing.
- Automatic removal of low-quality sequence data to improve assembly accuracy.
- The ability to use a reference sequence as a scaffold during assembly.
- Tools for generating restriction site maps, fragment lists, and open reading frame (ORF) maps.
- BLAST search functionality via integration with NCBI’s web services.
- Multiple alignment capabilities through ClustalW.
- Sequence manipulation features such as reverse complementation, exact or optimal sequence searching, translation display during nucleotide editing, and reverse translation plotting.
ChromasPro is ideal for molecular biologists, geneticists, and researchers involved in DNA sequencing projects. It provides a comprehensive set of tools to streamline sequence assembly, analysis, and visualization, enabling users to efficiently manage and interpret their data. ChromasPro can be installed via winget on compatible Windows systems.